Opto-/Chemogenetic
CAV PRS HA-hM4D(Gi)
An E1/E3-deleted, replication-defective, CAV-2 vector harbouring the PRS promoter that leads to the expression in noradrengenic neurons of hM4D(Gi) with an HA tag in N-Ter. The open reading frame is followed by an hGH polyA signal. Cassette is derived from plasmid #121538, Addgene.
CAV-2Opto-/ChemogeneticPRS promoter CAV-2 DREADD PRS promRead more
CAV PRS hM3D(Gq)-mCherry
An E1/E3-deleted, replication-defective, CAV-2 vector harbouring PRS promoter that leads to hM3D(Gq) fused to mCherry expression in noradrengenic neurons. The open reading frame is followed by an SV40 polyA signal. The vector was generated by the Pickering Lab (Bristol, UK).
CAV-2Opto-/ChemogeneticPRS promoter CAV-2 chemogenetic hM3D PRS promRead more
CAV PRS DIO hM4D(Gi)-mCherry
An E1/E3-deleted, replication-defective, CAV-2 vector harbouring the PRS promoter that leads to a Cre-dependent hM4D(Gi) fused to mCherry expression in noradrengenic neurons. The open reading frame is followed by an hGH polyA signal. Cassette derived from plasmid #44362, Addgene.
CAV-2Cre-inducibleOpto-/ChemogeneticPRS promoter CAV-2 chemogenetic PRS promRead more
CAV PRS stGtACR2-FRed
An E1/E3-deleted, replication-defective, CAV-2 vector harbouring PRS promoter that leads to soma-targeted Guillardia theta anion-conducting channelrhodopsin 2 fused to FusionRed FP expression in noradrengenic neurons. The open reading frame is followed by an SV40 polyA signal.
CAV-2Opto-/ChemogeneticPRS promoter CAV-2 optogenetic PRS promRead more
CAV hSyn DIO hM3D-mCherry
An E1/E3-deleted, replication-defective, CAV-2 vector harbouring the hSyn promoter that leads to a Cre-dependent hM3Dq fused to mCherry expression. The open reading frame is followed by an hGH polyA signal. Cassette derived from plasmid #44361, Addgene.
CAV-2Cre-inducibleOpto-/ChemogeneticRead more
CAV hSyn DIO hM4D-mCherry
An E1/E3-deleted, replication-defective, CAV-2 vector harbouring the hSyn promoter that leads to a Cre-dependent hM4Di fused to mCherry expression. The open reading frame is followed by an hGH polyA signal. Cassette derived from plasmid #44362, Addgene.
CAV-2Cre-inducibleOpto-/ChemogeneticRead more
CAV hM3D IRES mCitrine
An E1/E3-deleted, replication-defective, CAV-2 vector harbouring the CMV promoter that leads to hM3D (DREADD) and mCitrine expression. The open reading frame is followed by an SV40 polyA signal.
CAV-2Opto-/Chemogenetic DREADD fluorescent hM3DRead more
CAV DIO hM4D-mCherry
An E1/E3-deleted, replication-defective, CAV-2 vector harbouring the CMV promoter that leads to Cre-inducible hM4D (DREADD) fused to mCherry expression. The open reading frame is followed by an SV40 polyA signal.
CAV-2Cre-inducibleOpto-/Chemogenetic Cre inducible DREADD fluorescent hM4DRead more